Monthly Archives: February, 2007
Electrode Filling
Well, today puts things again in the realm of micropipette prep, and again things are not coming out precisely as expected. I’ve found that even though I thought previously that backfilling was not taking place in the pipettes that were finely tipped, apparently it is. The pipettes do not seal off on the end, even [...]
Pulling Electrodes & Other Musings
What I thought initially would be hard, proved to be exceptionally easy then exceptionally impossible. I’d heard from others long before I’d gotten into the business of pulling my own electrodes that this would be a road fraught with dismay and frustration, or at least fraught with cursing and many discarded electrodes that did not [...]
Electrophysiology
So, I’ve recently been training up on doing electrophysiology in the lab, and I thought I’d blog about the experiences a bit (mainly technical details) as I go along since I’ve encountered a number of issues along the way, and some I’ve resolved so far, whereas others still need some work to get going.
This is [...]
